| 质粒名称: | Thy1-Brainbow-1.1 M |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 18726 |
| 存储实验室: | Joshua Sanes |
|
载体骨架基本信息 |
|
| 载体名称: | pUC18+Thy1 promoter |
| 空载体大小: | 9188 bp |
| 完整质粒大小: | 13701bp |
| 载体类型: | Mammalian Expression, Mouse Targeting, Cre/Lox |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 未知 |
|
插入基因信息 |
|
| 插入基因名称: | Kusabira ; M-mCherry ; M-mEYFP ; M-mCerulean |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | NheI / XhoI (destroyed during cloning) |
| 3’克隆位点: | SspI / XhoI (destroyed during cloning) |
| 5’测序引物 | Thy1 F1 primer (TCTGAGTGGCAAAGGACCTTAGG) |
| 3’测序引物 | SV40pA-R |
|
文献引用方法 |
|
| 材料和方法部分: | Thy1-Brainbow-1.1 M was a gift from Joshua Sanes (Addgene plasmid # 18726 ; http://n2t.net/addgene:18726 ; RRID:Addgene_18726) |
| 参考文献部分: | Transgenic strategies for combinatorial expression of fluorescent proteins in the nervous system. Livet J, Weissman TA, Kang H, Draft RW, Lu J, Bennis RA, Sanes JR, Lichtman JW. Nature. 2007 Nov 1. 450(7166):56-62. 10.1038/nature06293 PubMed 17972876 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS Thy1-Brainbow-1.1 M 13701 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS Thy1-Brainbow-1.1 M
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 13701)
AUTHORS Livet J, Weissman TA, Kang H, Draft RW, Lu J, Bennis RA, Sanes JR,
Lichtman JW
TITLE Transgenic strategies for combinatorial expression of fluorescent
proteins in the nervous system.
JOURNAL Nature. 2007 Nov 1. 450(7166):56-62.
PUBMED 17972876
REFERENCE 2 (bases 1 to 13701)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..13701
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(10..28)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 96..200
/gene="bla"
/label=AmpR promoter
CDS 201..1061
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(419..438)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 1232..1820
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 1721..1740
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 1974..1991
/label=L4440
/note="L4440 vector, forward primer"
protein_bind 6253..6286
/label=loxN
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GTATACCT). loxN sites are compatible with each
other, but incompatible with loxP or lox2272 sites."
protein_bind 6294..6327
/label=lox2272
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GGATACTT). lox2272 sites are compatible with each
other, but incompatible with loxP or loxN sites (Lee and
Saito, 1988)."
protein_bind 6337..6370
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
regulatory 6384..6393
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 6390..7046
/codon_start=1
/product="Kusabira-Orange fluorescent protein (Karasawa et
al., 2004)"
/label=Kusabira-Orange
/translation="MVSVIKPEMKMKYFMDGSVNGHEFTVEGEGTGKPYEGHQEMTLRV
TMAKGGPMPFSFDLVSHTFCYGHRPFTKYPEEIPDYFKQAFPEGLSWERSLQFEDGGFA
AVSAHISLRGNCFEHKSKFVGVNFPADGPVMQNQSSDWEPSTEKITTCDGVLKGDVTMY
LKLAGGGNHKCQFKTTYKAAKKILKMPQSHFIGHRLVRKTEGNITELVEDAVAHC"
polyA_signal 7169..7250
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
protein_bind 7288..7321
/label=loxN
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GTATACCT). loxN sites are compatible with each
other, but incompatible with loxP or lox2272 sites."
regulatory 7442..7451
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 7448..8158
/codon_start=1
/product="monomeric derivative of DsRed fluorescent protein
(Shaner et al., 2004)"
/label=mCherry
/note="mammalian codon-optimized"
/translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
EGRHSTGGMDELYK"
primer_bind complement(7593..7611)
/label=mCherry-R
/note="mCherry, reverse primer"
primer_bind 7861..7880
/label=mCherry-F
/note="mCherry, forward primer"
polyA_signal 8286..8407
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
protein_bind 8490..8523
/label=lox2272
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GGATACTT). lox2272 sites are compatible with each
other, but incompatible with loxP or loxN sites (Lee and
Saito, 1988)."
regulatory 8642..8651
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 8648..9367
/codon_start=1
/product="enhanced YFP with monomerizing A206K mutation
(Zacharias et al., 2002)"
/label=mEYFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
polyA_signal 9490..9611
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
protein_bind 9626..9659
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
regulatory 9782..9791
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 9788..10507
/codon_start=1
/product="enhanced monomeric variant of CFP (Rizzo et al.,
2004)"
/label=mCerulean
/note=" mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
protein_bind 10550..10583
/label=FRT (minimal)
/bound_moiety="FLP recombinase from the Saccharomyces
cerevisiae 2u plasmid"
/note="supports FLP-mediated excision but not integration
(Turan and Bode, 2011)"
polyA_signal 10607..10728
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(13532..13551)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 13651..13673
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
ORIGIN
1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
901 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
2041 cgattcatta atgcaggatc gcggccgcga tccccgggcg agctcgaatt cagagaccgg
2101 gaaccaaact agcctttaaa aaacataagt acaggagcca gcaagatggc tcagtgggta
2161 aaggtgccta ccagcaagcc tgacagcctg agttcagtcc ccacgaacta cgtggtagga
2221 gaggaccaac caactctgga aatctgttct gcaaacacat gctcacacac acacacacaa
2281 atagtataaa caattttaaa tttcatttaa aaataatttg taaacaaaat cattagcaca
2341 ggttttagaa agagcctctt ggtgacatca agttgatgct gtagatgggg tatcattcct
2401 gaggacccaa aaccgggtct cagcctttcc ccattctgag agttctctct tttctcagcc
2461 actagctgaa gagtagagtg gctcagcact gggctcttga gttcccaagt cctacaactg
2521 gtcagcctga ctactaacca gccatgaaga aacaaggagt ggatgggctg agtctgctgg
2581 gatgggagtg gagttagtaa gtggccatgg atgtaatgac cccagcaatg ctggctagaa
2641 ggcatgcctc ctttccttgt ctggagacgg aacgggatca tcttgtactc acagaaggga
2701 gaacattcta gctggttggg ccaaaatgtg caagttcacc tggaggtggt ggtgcatgct
2761 tttaactcca gtactcagga ggcagggcca ggtggatctc tgtgagttca agaccagcct
2821 gcactatgga gagagttttg ggacagccag agttacacag aaaaatcctg gtggaaaatc
2881 tgaaagaaag agagaaagaa agaaagaaag aaagaaagaa agaaagaaag aaagaaagaa
2941 agaaagaaag aaagaaagga agaaagaaag aaagagtggc aggcaggcag gcaggaggaa
3001 ggaaggaagg aaggaaggaa ggaaggaagg aaggaaggaa ggaaggaagg aaggaaggaa
3061 aataggtgcg acttcaagat ccggagttac aagcagaatg cactgtttcc ctaacagggc
3121 caagtgtttt gagtaactga aggtgggcat gatgcctggg aagcagaaac aagccaggca
3181 gatgcacccc ttgccttgct tccgaagggc tgcagtagca tggaaaacat ggaaaacaac
3241 caatccattc cctttgctga tataacaggc tccaaagcca aaacctgtca ctggaggctc
3301 aagagcagat ctccagccaa gaggcaaagg aatgggggaa gctggagggc ctccctctgg
3361 ttatccaggc ttctgaaggt tcaagcaaag aaagggttac aaccttaaaa ggagagcgtc
3421 ccggggtatg ggtagaagac tgctccaccc cgacccccag ggtccctaac cgtcttttcc
3481 ctgggcgagt cagcccaatc acaggactga gagtgcctct ttagtagcag caagccactt
3541 cggacaccca aatggaacac ctccagtcag ccctcgccga ccaccccacc ccctccatcc
3601 ttttccctca gcctccgatt ggctgaatct agagtccctc cctgctcccc cctctctccc
3661 cacccctggt aaaactgcgg gcttcaggct gggtgcagca actggaggcg ttggcgacca
3721 ggaggaggct gcagctaggg gagtccaggt gagagcaggc cgacgggagg gacccgcaca
3781 tgcaaggacc gccgcagggc gaggatgcaa gccttcccca gctacagttt tgggaaagga
3841 taccagggcg ctcctatatg ggggcgcggg aacctgggga aagaaggtgc tcccaggtcg
3901 aggtgggaga ggaaggcagt gcggggtcac gggctttctc cctgctaacg gacgctttcg
3961 aagagtgggt gccggagaga accatgagga aggacatcaa ggacagcctt tggtccccaa
4021 gctcaaatcg ctttagtggt gcgaatagag ggaggaggtg ggtggcaaac tggagggagt
4081 ccccagcggt gacctcgtgg ctggctggtg cggggcacca ggtaagaaac gcaatgttgc
4141 gggaggggac tgggtggcag gcgcggggga ggggaaagct agaaaggatg cgagggagcg
4201 gaggggggag ggagcggggg aatctcaact ggtagaggaa agttaaaatg aggaaatagc
4261 atcagggtgg ggttagccaa gccgggcctc agggaaaggg cgcaaagttt gtctgggtgt
4321 gggcttaggt gggctgggta tgagattcgg ggcgccgaaa acactgctgc gcctctgcca
4381 aatcacgcta cccctgtatc tagttctgct aggcttctcc agccccagcc ccaattcttt
4441 tctcagtgtc cccttccctc ccctgaatct caagcccaca ctccctcctc cataacccac
4501 tgttatcaaa tctaagtcat ttgccaccca acaaccatca ggaggcggaa gcagacggga
4561 ggagtttgag atcaacttgg gctacatcac gagttccaag ctcaccaagg cttcttaagg
4621 agaccttgtc tctaaaatta attaattaat taattaatag tcccctttct ctgccacaga
4681 accttgggat ctggctcctg gtcgcagctc cccccacccc aggctgacat tcactgccat
4741 agcccatccg gaaatcctag tctattcccc atggatcttg aactgcagag agaatggcag
4801 agtggcccgc cctgtgcaaa ggatgttcct agcctaggtg gagctcgcga actcggagac
4861 tgtgcctctc ttgggcaagg acaggctaga cagcctgccg gtgttgagct agggcactgt
4921 ggggaaggca gagaacctgt gcagggcacg caatgaacac aggaccagaa aactgcagcc
4981 ctaggaacac tcaagagctg gccatttgca agcatctctg gcctccgtgc ttctcactca
5041 tgtcccatgt cttatacagg cctctgtggc acctcgcttg cctgatctca tccctagccg
5101 ttaagctttc tgcatgactt atcacttggg gcataatgct ggatacctac cattttctta
5161 gaccccatca aaatcctatt tgagtgtacg gttcggagaa ctccttattt atccggtaaa
5221 tgtcttttac tctgctctca gggagctgag gcaggacatt cctgagatac attgggagag
5281 gaatacagtt tcaataaaat aataggttgg gtggaggtac atgcctataa tgccaccact
5341 caggaaatgg tggcagcttc gtgagtttga ggccaaccca agaaacatag tgaaaccctg
5401 tcagtaaata agtaagcaag tatttgagta tctactatat gctagggctg acctggacat
5461 taggggtcat cttctgaaca aactagtgct tgagggaggt atttggggtt tttgtttgtt
5521 taatggatct gaatgagttc cagagactgg ctacacagcg atatgactga gcttaacacc
5581 cctaaagcat acagtcagac caattagaca ataaaaggta tgtatagctt accaaataaa
5641 aaaattgtat tttcaagaga gtgtctgtct gtgtagccct ggctgttctt gaactcactc
5701 tgtagaccag gctggcctgg aaatccatct gcctctgcct ctctgcctct ctgcctctct
5761 gcctctctct ctgcctctct ctgcctctct ctgcccctct ctgcccctct ctgcccctct
5821 ctgccgccct ctgccttttg ccctctgccc tctgttctct ggcctctgcc ctctccctgc
5881 cttctccctc tgcctctgcc tcttgagtgc tggaatcaaa ggtgtgagct ctataggtct
5941 taagttccag aagaaacgta atgaagtcac ccagcaggag gtgctcaggg acagcaagac
6001 acacacaccc aggacactag gctcccactt ccttggcttt ctctgagtgg caaaggacct
6061 taggcagtgt cactccctaa gagaagggga taaagagagg ggctgaggta ttcagtcatg
6121 tgctccgtgg atctcaagcc ctcaaggtaa atggggaccc acctgtccta ccagctggct
6181 gacctgtagc tttccccacc acagaatcca agtcggaact cttggcacct agaggatctc
6241 gactagctcg agataacttc gtatagtata ccttatacga agttatcgag ctcataactt
6301 cgtataggat actttatacg aagttataac gtcgagataa cttcgtatag catacattat
6361 acgaagttat accggcagat ctcgccacca tggtgagtgt gattaaacca gagatgaaga
6421 tgaagtactt catggacggc tccgtcaatg ggcatgagtt cacagttgaa ggtgaaggca
6481 caggcaaacc ttacgaggga catcaagaga tgacactacg cgtcacaatg gccaagggcg
6541 ggccaatgcc tttctcgttt gacttagtgt cacacacgtt ctgttacggc cacagacctt
6601 ttactaaata tccagaagag ataccagact atttcaaaca agcatttcct gaaggcctgt
6661 catgggaaag gtcgttgcag ttcgaagatg gtgggttcgc tgccgtcagt gcgcatataa
6721 gccttagagg aaactgcttc gagcacaaat ccaaatttgt tggggttaac tttcctgccg
6781 atggtcctgt gatgcaaaac caaagttctg attgggagcc atcaaccgag aaaattacta
6841 cctgcgacgg agttctgaag ggtgatgtta cgatgtacct aaaacttgcg ggaggcggca
6901 atcacaaatg ccaattcaag actacttaca aggcggcaaa aaagattctt aaaatgccac
6961 aaagccattt catcgggcat cgcctcgtca ggaaaaccga aggcaacatt actgagctgg
7021 tagaagatgc agtagctcat tgctgaagcg gccgcgactc tagatcataa tcagccatac
7081 cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa
7141 acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa
7201 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
7261 tggtttgtcc aaactcatca atgtatcata acttcgtata gtatacctta tacgaagtta
7321 taggatctcc gccaccatgc tgtgctgcat cagaagaact aaaccggttg agaagaatga
7381 agaggccgat caggagctgc agtcgacggt accgcgggcc cgggatccac cggtagcatc
7441 cgccaccatg gtgagcaagg gcgaggagga taacatggcc atcatcaagg agttcatgcg
7501 cttcaaggtg cacatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg
7561 cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca agggtggccc
7621 cctgcccttc gcctgggaca tcctgtcccc tcagttcatg tacggctcca aggcctacgt
7681 gaagcacccc gccgacatcc ccgactactt gaagctgtcc ttccccgagg gcttcaagtg
7741 ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc gtgacccagg actcctccct
7801 gcaggacggc gagttcatct acaaggtgaa gctgcgcggc accaacttcc cctccgacgg
7861 ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc tccgagcgga tgtaccccga
7921 ggacggcgcc ctgaagggcg agatcaagca gaggctgaag ctgaaggacg gcggccacta
7981 cgacgctgag gtcaagacca cctacaaggc caagaagccc gtgcagctgc ccggcgccta
8041 caacgtcaac atcaagttgg acatcacctc ccacaacgag gactacacca tcgtggaaca
8101 gtacgaacgc gccgagggcc gccactccac cggcggcatg gacgagctgt acaagtaaac
8161 acgtgcggcc gcgactctag atcataatca gccataccac atttgtagag gttttacttg
8221 ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg
8281 ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
8341 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
8401 tatcttaagg cgtaaattgt aagcgttaat gctccgaggg ccgccaccac ctgttcctgt
8461 aggaattggc cgcgactcta ggatctagga taacttcgta taggatactt tatacgaagt
8521 tattgatcaa ttcacagcca ccatgctgtg ctgcatcaga agaactaaac cggttgagaa
8581 gaatgaagag gccgatcagg agctgcagtc gacggtaccg cgggcccggg atccaccggt
8641 cgccaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
8701 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
8761 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
8821 gcccaccctc gtgaccacct tcggctacgg cctgcagtgc ttcgcccgct accccgacca
8881 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
8941 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
9001 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
9061 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca
9121 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca
9181 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
9241 caaccactac ctgagctacc agtccaagct gagcaaagac cccaacgaga agcgcgatca
9301 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
9361 caagtaaagc ggccgcgact ctagatcata atcagccata ccacatttgt agaggtttta
9421 cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt
9481 gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
9541 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
9601 aatgtatctt aacgcgcgat tcgagataac ttcgtatagc atacattata cgaagttata
9661 ccggaattaa ttcacagcca ccatgctgtg ctgcatcaga agaactaaac cggttgagaa
9721 gaatgaagag gccgatcagg agctgcagtc gacggtaccg cgggcccggg atccaccggt
9781 cgccaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
9841 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
9901 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
9961 gcccaccctc gtgaccaccc tgacctgggg cgtgcagtgc ttcgcccgct accccgacca
10021 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
10081 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
10141 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
10201 ggggcacaag ctggagtaca acgccatcag cgacaacgtc tatatcaccg ccgacaagca
10261 gaagaacggc atcaaggcca acttcaagat ccgccacaac atcgaggacg gcagcgtgca
10321 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
10381 caaccactac ctgagcaccc agtccaagct gagcaaagac cccaacgaga agcgcgatca
10441 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
10501 caagtaaagc ggccgcgact ctagatcacc gcgactctag atcacgcgcg aagttcctat
10561 tctctagaaa gtataggaac ttctcgagta ctcaattgtt gttgttaact tgtttattgc
10621 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
10681 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttaag gcgtaaattg
10741 taagcgttaa ttcgaggtcc ttcctctgca gaggtcttgc ttctcccggt cagctgactc
10801 cctccccaag tccttcaaat atctcagaac atggggagaa acggggacct tgtccctcct
10861 aaggaacccc agtgctgcat gccatcatcc cccccaccct cgcccccacc cccgccactt
10921 ctccctccat gcataccact agctgtcatt ttgtactctg tatttattct agggctgctt
10981 ctgattattt agtttgttct ttccctggag acctgttaga acataagggc gtatggtggg
11041 taggggaggc aggatatcag tcccctgggg cgagttcctc cctgccaacc aagccagatg
11101 cctgaaagag atatggatga gggaagttgg actgtgcctg tacctggtac agtcatactc
11161 tgttgaaaga atcatcgggg aggggggggg gctcaagagg ggagagctct ctgagccttt
11221 gtggaccatc caatgaggat gagggcttag attctaccag gtcattctca gccaccacac
11281 acaagcgctc tgccatcact gaagaagccc cctagggctc ttgggccagg gcacactcag
11341 taaagatgca ggttcagtca gggaatgatg gggaaagggg taggaggtgg gggagggatc
11401 accccctcct ctaaaacacg agcctgctgt ctccaaaggc ctctgcctgt agtgagggtg
11461 gcagaagaag acaaggagcc agaactctga ctccaggatc taagtccgtg caggaagggg
11521 atcctagaac catctggttg gacccagctt accaagggag agcctttatt ccttctttcc
11581 cttgcccctc tgtgccagcc cctcttgctg tccctgatcc cccagacagc gagagtcttg
11641 caacctgcct cttccaagac ctcctaatct caggggcagg cggtggagtg agatccggcg
11701 tgcacacttt ttggaagata gctttcccaa ggatcctctc ccccactggc agctctgcct
11761 gtcccatcac catgtataat accaccactg ctacagcatc tcaccgagga aagaaaactg
11821 cacaataaaa ccaagcctct ggagtgtgtc ctggtgtctg tctcttctgt gtcctggcgt
11881 ctgtctcttc tgtgttcttc caaggtcaga aacaaaaacc acacacttca acctggatgg
11941 ctcggctgag cacttctgtg tgcagaaggt ccaaccagac tctggggtac cccggccctc
12001 cctattccct tgcctcctgt ctcccgcttt ttatagctcc ctatgctggg cttctctgga
12061 gagtgaaatc tttgcccaaa tcaatgcgca ttctctctgc tgagtcatct ggcgacagca
12121 gttgagttca cccgccaaca catgggccca gctatgtagc cgaaccctgg ctctggaagt
12181 gccagggact ttgtgcataa gtatgtacca tgcccttttt tcacagtcct agctctgcag
12241 aagtgcagcc tgaaggcctg tctgctgaga ggacatgccc tggagccctg aaacaggcac
12301 agtgggagga ggaacggagg atgacaggca tcaggccctc agtccaaaag caaccacttg
12361 agaatgggct ggagtacgaa acatggggtc ccgtccctgg atccctcctc aaagagtaat
12421 aagtaaaata taaacaggta ccccaggccg ttctgggttt gggttgtaat gggatccatt
12481 tgcagagaac tattgagaca gcccagccgt actgtgacag gcaatgtggg ggaggaggtt
12541 gaatcacttg gtatttagca tgaatagaat aattccctga acatttttct taaacatcca
12601 tatctaaatt accaccactc gctcccagtc ttcctgcctt tgcgccagcc tcctgtctgg
12661 ccatgcctga agaaggctgg agaagccacc cacctcaggc catgacactg ccagccactt
12721 ggcaggtgca gccaaacctg agctgtccca gaaagggaca ttctcaagac ccaggcaccc
12781 tgatcagcac tgacttggag ctacaagtgt catgccagaa aagtctctaa gaaaaccttt
12841 tcagggaaaa gggggtgact caacaccggg caagtttggg aagccccacc cttcgagtga
12901 tggaagagca gataggaagc ctcagaagag agacaccggc acccaggtaa cgttcctcat
12961 gtggtctctg tcacactagg tgctcttccc tggacatctc cgtgaccaca ctctcagttc
13021 ttagggagat gcgggtgctc tctgaggcta tctcagagtt gcagattctg aggcctagag
13081 tgactacagt cagcctagga agccacagag gactgtggac caggagggca gaagaggaga
13141 agggaagaaa aaccatcaga taggacttgc aatgaaacta acccaagaca atcataatgc
13201 agacaggaat gttaaaggcg ttcagcagct ggccatgaca cccatctgtc cctctggcca
13261 agtcagcaag cctggaagac ctgggactcc tgcccatatg tcctaagctc cccacccacc
13321 cactcgttca ctgtccttat tctctctcta ccttcagcca cttagtttcc taccttaagt
13381 cctagaattg atcctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
13441 tgcgcagcct gaatggcgaa tggcgcctga tgcggtattt tctccttacg catctgtgcg
13501 gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
13561 gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
13621 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
13681 cgtcatcacc gaaacgcgcg a
//
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